Serologic Evidence of Nipah Virus Infection in Bats, Vietnam

To the Editor: Bats are potential reservoir for highly pathogenic viruses, such as Nipah virus (NiV) and Hendra virus, which can cross species barriers (1). However, only limited surveillance has been conducted to assess risk for infection by these deadly emerging viruses. We conducted a study in Vietnam from 2007 to 2008 to assess the prevalence of these pathogens in bats. Different species of live bats were obtained from hunters or were captured in caves, pepper fi elds, and residential areas by using mist nets or harp traps (online Technical Appendix Figure 1, wwwnc.cdc.gov/ EID/pdfs/11-1121-Techapp.pdf). A total of 451 serum samples were collected and subjected to IgG ELISA by using an Escherichia coli–derived recombinant nucleocapsid (N) protein of NiV (NiV-N-ELISA) (2). Two Leschenault’s rousette bats (Rousettus leschenaulti) were vaccinated with the same recombinant N protein to obtain positive serum specimens that contained antibodies against NiV-N. An optical density of 492 nm for negative control serum (1,000× dilution) was designated as 1:1,000 ELISA units. ELISA titers of sample serum specimens were obtained at a single dilution (1,000×) by using a standard curve of positive serum with high titers. A sample titer >3,000 was considered positive for IgG against NiV. Positive results were detected from only 2 fruit bat species, R. leschenaulti (31 bats [49.1%]) and Cynopterus sphinx (3 bats [2.8%]) (Table). Of the 34 samples positive by ELISA, only 22 (20 from the former and 2 from the latter bat species), which had enough volume left, were further analyzed by Western blot (WB) with E. coli–expressed recombinant N protein of NiV (Table; online Technical Appendix Figure 2). Twenty of the 22 specimens were confi rmed as positive by WB. ELISApositive samples with high titers were also positive by WB for both bat species. However, only 1 sample from an R. leschenaulti bat was positive by WB that used a baculovirus-expressed recombinant N protein. Because of the different protein expression systems, the reactivity of bat antibody against NiV protein in WBs showed different patterns (online Technical Appendix Figure 2). Neutralization tests (NTs) in which live NiVs (strain Ma-JMR-01–98) were used were performed at the Institute of Tropical Medicine, Nagasaki University. No specimens of C. sphinx bats were positive by NT; however, 2 specimens from R. leschenaulti bats, both positive with low titers, were confi rmed to be positive by NT (50% cytopathic effect after NT, titers of 33.6 and 14.1). However, the latter specimen was negative by WB analysis. Seroepidemiologic studies in other countries have indicated that Pteropus spp. bats (fruit-eating bats) are the main reservoirs for NiV (3–6). Pteropid bats are usually found only in southern Vietnam. We could not obtain these bats for our study. However, a relatively high prevalence (49.1%) of henipavirus antibody was found in R. leschenaulti specimens from Hoa Binh Province. Rousettus spp. bats are the only megabats that use echolocation. These bats hang together on cave ceilings in a tightly packed manner and in groups composed of bats of both sexes and of different ages. They roost in large colonies and fl y vast distances to fi nd fruit (7). This behavior might be related to their high rate of seropositivity for viral infections. In southern China, bats of the same species showed a high prevalence of henipavirus antibody (8). R. leschenaulti bats are distributed from central to northern Vietnam and southern China. C. sphinx bats are common all over Vietnam, and their